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| Medical Professionals > Departments
> Ancillary Services |
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| Introduction |
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ICMR National Tumour Tissue Repository (INTTR) is the first Tumour Tissue Repository in India and was established with the support from Indian Council of Medical Research (ICMR)
We collect and preserve disease as well as normal tissues and blood for prospective research initiative as an organized bank .
Over 14,000 samples have been collected and catalogued by the repository till date
( 28 / 02 / 2010)
Our Mission is
- To establish methods to collect and preserve disease as well as normal tissues and blood for prospective research initiative as an organized bank
- To supply adequate quantities of high quality research grade tissues to individual investigators for their IRB approved projects
- To maintain and provide clinical information to the individual Investigators.
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| Services |
Tissue Collection & Preservation
Steps involved in providing INTTR's standard services are as follows 
- Informed Consent
- Collection of Biospecimens
- Snap Freezing & Coding
- Storage
- Tissue Repository Data Base
Additional Services offered by INTTR
- Temporary Storage of Biospecimens (Hotel Services)
- Follow Up Data
Informed Consent
Informed consent is administered to the patient preoperatively with the help of surgery residents and nurses. All the care is taken by INTTR staff to protect the patients’ interest and rights.
Collection of the Specimens
 Tissue is for the diagnosis first. Only surplus tissue is collected for the repository without compromising histopathologic diagnosis.
The freshly delivered operative specimen (tumour and normal) is incised with different sterile scalpel blades (for tumour and normal) and quickly a portion of tissue (aprox. 1 cubic cm ) is removed under the supervision of surgeon in the operation theatre / or pathologist in the frozen section room under sterile conditions.
Virtually all types of tissues are collected except necrotic tissues from every organ and site, of every histology, regardless of prior treatment status and regardless of any infective status
If possible, blood is also collected simultaneously during the patients’ operative procedure in OT by the anesthetist. The blood, so collected, is processed to separate serum, plasma & buffy coat & separated components are then frozen.
Snap Freezing & Coding
Tissues are collected in cryovials and rapidly snap frozen in liquid nitrogen in a portable Dewar flask within 5 minutes so as to prevent degradation of RNA and protein. Time from tissue excision to freezing is noted in the data base of INTTR.
Cryovials are coded so as to maintain confidentiality of the patients.
Storage
 Cryovials are stored in liquid nitrogen containers or -80 0C deep freezers.
Tissue Repository Data base
The repository collection includes a variety of tumour tissues, as well as normal tissues and blood whenever available.
Site wise available tissues updated as on 28th February 2010
Anatomic Sites |
No. of cases |
| Breast & Axillary nodes |
2142 |
| Head / Neck & nodes |
4916 |
| Gastrointestinal Tract |
1099 |
| GU / GYN |
1918 |
| Thoracic lesions |
988 |
| Bone & Soft tissues |
531 |
Total |
11594 |
A computer program for easy accessibility, disbursement of specimens and follow up data is developed.The data base is linked to Hospital Diagnostic Information System.
Data base includes physical location of the sample, site wise availability of tissues and other basic demographic data.
Temporary storage of biospecimens ("Hotel" Services)
Deep freezer and liquid nitrogen storage facility of INTTR can be used for temporary storage of biospecimens by the investigators. Investigators will have to notify the INTTR regarding types of samples, title & IRB number of the project. Investigators will also be required to keep their own records of freezer location
Follow Up Data
Besides basic data of the samples requested, extra clinical data are also provided to the researchers as per their need.
Follow up data are retrieved from the Medical Records Office by INTTR staff
Quality Control
Representativeness of the lesion and histopathologic diagnosis of collected tissues is assessed by histopathologist and integrity of tissues (nucleic acid quality and quantity) is checked by Molecular Pathology Laboratory. The investigators are asked to fill up feedback form and their comments/suggestions are sought.
To download a Requisition Form, Please click here
For TMC Investigators;
Please note that samples will be issued only to IRB approved projects. Attach a copy of IRB approval along with the Requisition Form.
For requests from other institutes:
Please send duly filled & signed Material Transfer Agreement Form along with the Requisition Form and a soft copy of Project Proposal for which tissues are being asked for.
For Material Transfer Agreement Form, Please click here
Send your duly filled and signed Requisition Form / Material Transfer Agreement Form to
ICMR National Tumour Tissue Repository
5th Floor, Annexe Building,
Tata Memorial Hospital,
Dr E Borges Marg,
Parel, Mumbai- 400 012
Or
Scan and Mail to: ttrtmh@gmail.com
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| Charges |
INTTR is nonprofit activity. In order to sustain the facility, the following charges are levied to non-TMC investigators
- Tissue/Serum/ Plasma or Buffy coat sample – Rs 500/ per vial
Payments should be made only in the form of Demand Draft in favour of Tumour Tissue Repository, Tata Memorial Centre payable at Mumbai |
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| Ethics |
Tissue is for diagnosis first. Only surplus tissue is given to the repository without compromising the histopathologic diagnosis.
No tissue is collected/stored in the INTTR without administration of informed consent .
Samples are assigned a specific INTTR number. Identity of the donor is not revealed to the researcher to maintain confidentiality of sensitive research data.
Rights of donors are protected. Patient has right to withdraw his / her tissue from the repository at any point of time. Standard safeguards for accession, collection, storage and disbursement of tissues are followed as per the ethical guidelines.
Tissues are disbursed only when a copy of IRB approval of project is enclosed with the Requisition Form. |
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| Contact Us |
1 ICMR National Tumour Tissue Repository
5th Floor, Annexe Building,
Tata Memorial Hospital,
Dr E Borges Road,
Parel, Mumbai – 400 012. India
Telephone : 91-22- 24177000 Extension- 4359
E mail: ttrtmh@gmail.com
2. Dr Sangeeta Desai / Ms. Manisha Kulkarni,
Department of Pathology,
Tata Memorial Hospital
Telephone: 91-22 -21417273
91-22-24177000 ext 4385
E mail: sangeetabdesai@rediffmail.com, manishabkulkarni@gmail.com |
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Tata Memorial Hospital, Dr.
E Borges Road, Parel, Mumbai - 400 012 India. 